chuka_lis (chuka_lis) wrote,


Немецкие ученые выяснили, что аллицин (компонент чеснока) в культурах клеток препястствует репликации коронавируса и заражению клеток. Он стимулирует гены, влияющие на производство интерферона, транскрипцию и трансляцию в человеческой клетке.
Правда, доза должна быть умеренной, тк это токсичный компонент, и, на мой взгляд, лучше применять чеснок не на уровне развития пневмонии и ТОРС, а на уровне ОРЗ в верхних дыхательных путях. Впрочем, если придумают рабочий способ ингаляции, то лишь бы помогало.
Apart from drugs, functional food based on herbal medicine is suggested as prophylaxis to prevent COVID-19 outcome due to their immunomodulatory, antioxidant, anticancer, antimicrobial and antiviral activities [4, 8]. For example, garlic plants (Allium sativum) produce volatile organosulfur compounds, such as diallyl thiosulfinate (allicin) and diallyl polysulfanes, which are known to stimulate the immune system by modulation of cytokine secretion and pro-inflammatory cytokines [4, 8, 9]. Allicin showed broad-spectrum antimicrobial activity against several pathogenic bacteria, viruses, fungi and parasites and has been used for the treatment of pneumonia, tuberculosis and the common cold since ancient times [9-17]. Administration of the volatile allicin by inhalation can efficiently reach the infected lung tissue.Allicin is a strongly thiol-reactive compound, which reacts with Cys thiols via thiol-disulfide exchange reactions, leading to S-thioallylations of proteins [19, 20].In Jurkat cancer cells, 332 S-thioallylated proteins were identified 10 min after allicin treatment, including highly abundant cytoskeleton proteins, HSP90 chaperones, translation elongation factors and glycolytic enzymes. A llicin caused disruption of the actin cytoskeleton, enzymatic inactivation and Zn2+ release to stimulate the IL-1-dependent IL-2 secretion by T-cells as an immunomodulatory effect [22]Since SARS-CoV-2 is rich in Cys residues in its surface spike glycoprotein, a reduced state of the host cell cytoplasm is required for efficient virus entry and membrane fusion. Moreover, allicin is cell permeable and has been shown to cause transient pore formation in phospholipid membranes, which may contribute to the killing of SARS-CoV-2 by affecting its envelope [20, 26]. While the antiviral effect of allicin has been studied against several viruses that cause respiratory tract infections, including influenza, SARS-CoV and rhinovirus [16], mechanistic insights on its proposed antiviral effects against SARS-CoV-2 in the infected host cell are lacking.
..the sub-lethal biocompatible doses of allicin were determined as 50-75 μM in Vero E6 cells and 100-200 μM in the more tolerant Calu-3 cells.
..results suggest that allicin affects host-pathogen interactions by antiviral and immunomodulatory activities. We further investigated viral replication after allicin exposure by determination of viral RNA genome equivalents (GE) from the supernatant of infected cells using quantitative RT-PCR. In agreement with the plaque assays, the qRT-PCR results revealed a 72% lower amount of viral RNA after addition of 50 μM allicin to SARS-CoV-2 infected Vero E6 cells. Moreover, virus plaque assays and qRT-PCR results showed an almost complete >99 % inhibition of SARS-CoV-2 replication after exposure to 75 μM allicin, supporting the strong antiviral activity of allicin in infected Vero E6 cells
Additionally, the antiviral effects of biocompatible doses of allicin were analyzed in the human lung cell line Calu-3. After infection with SARS-CoV-2 at a multiplicity of infection (MOI) of 0.01 and 0.005 Calu-3 cells were treated with biocompatible doses of 100 and 200 μM allicin and analyzed 16 h p.i. and 24 h p.i., respectively (Fig. 3). Treatment of infected Calu-3 cells with 100 μM allicin did not significantly inhibit viral replication (Fig. 3A-D). However, exposure of infected Calu-3 cells to 200 μM allicin led to a significant >60 % decrease of viral RNA (Fig. 3A, B)and a >65% reduction of infectious particles.
he antiviral effect of allicin on SARS-CoV-2 infected Calu-3 cells was further supported by microscopy imaging. While SARS-CoV-2 infection at a MOI of 0.01 resulted in cellular damageof Calu-3 cells after 24 h p.i., the addition of allicin partially protected the cells against this damage .
Volatile garlic organosulfur compounds, such as the thiol-reactive allicin (diallyl thiosulfinate) exert strong antimicrobial activity against various respiratory pathogens. Here, we investigated the antiviral activity of allicin against SARS-CoV-2 in infected Vero E6 and Calu-3 lung cells. Allicin efficiently inhibited viral replication and infectivity in both cell lines. Proteome analyses of infected Calu-3 cells revealed a strong induction of the antiviral interferon-stimulated gene (ISG) signature (e.g. cGAS, Mx1, IFIT, IFIH, IFI16, IFI44, OAS and ISG15), pathways of vesicular transport, tight junctions (KIF5A/B/C, OSBPL2, CLTC1, ARHGAP17) and ubiquitin modification (UBE2L3/5), as well as reprogramming of host metabolism, transcription and translation. Allicin abrogated the ISG host response and reverted the host cellular pathways to levels of uninfected Calu-3 cells, confirming the antiviral and immunomodulatory activity of allicin in the host proteome. Thus, biocompatible doses of garlic could be promising for protection of lung cells against SARS-CoV-2.
Tags: коронавирус, лечение, статьи

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